Diselenide crosslinks for enhanced and simplified oxidative protein folding
نویسندگان
چکیده
منابع مشابه
Oxidative protein folding
During seed development, endosperm cells of highly productive cereals, including rice, synthesize disulfide-rich proteins in large amounts and deposit them into storage organelles. Disulfide bond formation involves electron transfer and generates H(2)O(2) as a by-product. To ensure proper development and maturation of seeds, the endosperm cells must supply large amounts of oxidizing equivalents...
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Although oxidative folding of disulfide-rich proteins is often sluggish, this process can be significantly enhanced by targeted replacement of cysteines with selenocysteines. In this study, we examined the effects of a selenosulfide and native versus nonnative diselenides on the folding rates and mechanism of bovine pancreatic trypsin inhibitor. Our results show that such sulfur-to-selenium sub...
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Ten years ago it was thought that disulphide bond formation in prokaryotes occurred spontaneously. Now two pathways involved in disulphide bond formation have been well characterized, the oxidative pathway, which is responsible for the formation of disulphides, and the isomerization pathway, which shuffles incorrectly formed disulphides. Disulphide bonds are donated directly to unfolded polypep...
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The endoplasmic reticulum (ER) provides an environment that is highly optimized for oxidative protein folding. Rather than relying on small molecule oxidants like glutathione, it is now clear that disulfide formation is driven by a protein relay involving Ero1, a novel conserved FAD-dependent enzyme, and protein disulfide isomerase (PDI); Ero1 is oxidized by molecular oxygen and in turn acts as...
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In eukaryotes, disulphide bond formation and isomerization is catalysed in the endoplasmic reticulum by protein disulphide isomerase (PDI) and related proteins. Although this eukaryotic system has been intensely studied for almost 40 years, many questions remain unanswered. In 1991, it was discovered that disulphide bond formation in the prokaryotic periplasm is also a catalysed process (Bardwe...
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ژورنال
عنوان ژورنال: Communications Chemistry
سال: 2021
ISSN: 2399-3669
DOI: 10.1038/s42004-021-00463-9